Composite

Part:BBa_K4336005:Design

Designed by: YI TING LEE   Group: iGEM22_NTHU_Taiwan   (2022-10-07)


[Ranalexin] E [Ranalexin]' E [Lysostaphin]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 70
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 70
    Illegal BamHI site found at 522
    Illegal XhoI site found at 877
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 70
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 70
    Illegal NgoMIV site found at 99
    Illegal NgoMIV site found at 547
    Illegal NgoMIV site found at 562
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of glutamate residue is linker. Inside the bracket is the sequence of the antimicrobial peptides. Inside the bracket with apostrophe is sequence edit with different codon of the same protein.


Source

It comes from combination of different antimicrobial peptides.

References